Invigor8 Reef must be adequately saturated with water prior to application; failure to saturate components will result in loss to surface skimming devices.  Depending upon method of application, system water or purified water may be utilized in saturation process; review entire information below.

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In a clean mixing vessel, mix in a ratio of ~10:1 water (mL) to Invigor8 Reef (g) (ex. mix 100 mL water with 10 g Invigor8 Reef).  If a thinner fluid consistency is desired, then use more water.  

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Cap and shake vessel to thoroughly saturate Invigor8 Reef components, and allow to sit for 1 - 2 minutes prior to use.  Prior to each use, shake vessel to re-suspend components.

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Determining mass of Invigor8 Reef required: If applying this formula in systems raising cohorts of suspension-feeding sessile invertebrates (e.g. cnidarians, poriferans, sedentarians, bivalvians, ascidians, etc.), then apply 0.20 g of Invigor8 Reef per square foot of colonized area per day.  Modify application frequency and mass of Invigor8 Reef applied per cohort requirements.

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Recommended: Determine mass of Invigor8 Reef required during a period of no more than 12 hours, then add this amount to a clean mixing vessel with adequate water from the system to form a suspension with the desired fluid consistency.  Allow vessel to remain at room temperature.  If using system water, then entire contents of vessel should be used within 12 hours of initial mixing.  This approach is recommended because the temperature of the suspension will closely match the system temperature, as opposed to using a suspension which is considerably colder due to refrigeration.  Target individual cohort members, or apply suspension in close proximity to circulation pump discharge, as appropriate.

 

Metered Application: If mixing a larger amount of Invigor8 Reef suspension than will be required for 12 hours, then substitute purified water for system water in the preceding instructions, and store Invigor8 Reef suspension at T≤40°F outside of periods of use (do not allow to freeze).  Pour required volume of mixture into syringe or separate dosing vessel. Do not contaminate mixture with system water.  The use of a magnetic stirring platform to maintain homogeneity of the suspension is recommended if application will be performed with a dosing pump.  Locate point of entry in close proximity to circulation pump discharge.  If applying manually, then shake vessel prior to application to ensure homogeneity of suspension.  Target individual cohort members, or apply suspension in close proximity to circulation pump discharge, as appropriate.